Tue, 05/12/2020 - 17:53
- A very nice paper by the group of Roger Legrand in Paris, showing that hydroxychloroquine (HCQ) used at a similar concentration as in humans has no prophylactic or therapeutic effect on SARS-CoV-2 infection in cynomolgus macaques. They also show that it is active in vitro on infection of Vero cells in the low µM range, but NOT in a human airway epithelium. The plasma concentration was below the IC50 value in Vero cells.
- Simplified and fast testing for SARS-CoV-2 based on CRISP-Cas (see 2 papers, editorial and review). These platforms use the following simplifications:
- Based on a swab or other sample RNA is extracted and reverse transcribed.
- Primers for specific regions in E or N are applied.
- No thermocycler (for PCR), but iso-thermal amplification strategies (just requiring a water bad), such as RPA (recombinase polymerase amplification) or LAMP (loop-mediated amplification) are used
- The CRISPR Cas 12 or 13 is used to recognize a specific sequence by a specific guide RNA. If this sequence is present, the Cas enzyme is activated and that will cleave a single stranded DNA reporter, which starts to fluoresce.
- The detection is done either in a plate reader or a lateral flow devise
- The whole procedure is very rapid (40 minutes) and has a high specificity and sensitivity.
See figure 8 p. 12 (Esbin), Fig 1 p.5 (Curti) or Fig 1 p.2 (Broughton).
18 Feb 2023 Episode 316: Under which circumstances could type I or type III IFN be a useful treatment?
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